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Chadrack Mbala Mulo was mute and autistic and was unable to feed himself or raise the alarm after his mum Esther suffered a fatal fit. Mum Esther was clearly as excited as any other when her little boy started his first week of school last September. Posting a picture of her four-year-old Chadrack in his uniform on Facebook mum, she added just one word: "Love". But barely a month later both of them were found dead in their flat in the most tragic of circumstances. Esther Eketi-Mulo is believed to have suffered an unexpected and fatal epileptic fit some time around October 1 or 2 last year. When the pair were finally discovered on October 20, little Chadrack was found still clinging to his mother's badly decomposed corpse.
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Acute febrile illness has a broad differential diagnosis and can be caused by a variety of pathogens. Metagenomic next-generation sequencing NGS is particularly attractive for diagnosis and public health surveillance of febrile illness because the approach can broadly detect viruses, bacteria, and parasites in clinical samples by uniquely identifying sequence data [ 1 , 2 ]. However, timely analysis using second-generation platforms such as Illumina and Ion Torrent has been hampered by the need to wait until a sufficient read length has been achieved for diagnostic pathogen identification, as sequence reads for these platforms are generated in parallel and not in series. The time differential is accounted for by increased times for library quantitation, sequencing, and bioinformatics analysis with the Illumina protocol. The stopwatch is depicted as a hr clock. Nanopore sequencing is a third-generation sequencing technology that has two key advantages over second-generation technologies — longer reads and the ability to perform real-time sequence analysis.
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